A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS
نویسندگان
چکیده
منابع مشابه
A Routine Technique for Double-staining Ultrathin Sections Using Uranyl and Lead Salts
For more than 2 years, a double-staining of sections of OsO 4-fixed and Epon-embedded tissues with uranyl acetate followed by lead hydroxide has been routine technique in this laboratory. It was found to give substantially higher contrast, thus permitting the use of thinner sections. It facilitated focusing and routinely provided clearer pictures of cell fine structure than could be obtained wi...
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Immersion of tissue in aqueous uranyl acetate be-for giving some degree of contrast to components fore embedding is commonly practiced to increase such as membranes when they extend through the the contrast on sections which are subsequently thickness of the section parallel to the electron stained (2, 3, 5). The treatment is only satisfactory beam. A simple modification of current practice 540
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Meta l impregnat ion , or "s ta in ing ," of tissue sections for electron microscopy has become the accepted practice in recent years. Salts of metals of h igh atomic weight such as u ran ium, chromium, thor ium, lead, or tungsten, among many tested, have been found suitable (6, 1). "Lead hydroxide ," as prepar ted by Watson (6), is now widely used, hu t this solution is extremely unstable in a...
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One of the potential advantages of high-voltage electron microscopes is that their greater penetrating power allows the use of thick sections (0.22 lt) to study three-dimensional cytoarchitecture . So far, this advantage has been limited because conventional methods of staining only allow the penetration of heavy metal solutions into sections to a depth of a few hundred Angstroms . For example,...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.25.1.157